Mycobacterium branderi Koukila-Kahkola et al.
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2. From a single tube of the recommended broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or
1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Heavily inoculate a slant of #90 and/or a plate of #173 agar.
5. Incubate at 37oC.
6. After growth has been achieved, additional transfers may be made.
Colonies on #173 plates are smooth, circular, entire, convex and buff to yellow in color.
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Koukila-Kahkola P, et al. Mycobacterium branderi sp. nov., a new potential human pathogen. Int. J. Syst. Bacteriol. 45: 549-553, 1995. PubMed: 8590682
Brander E, et al. Characterization of a distinct group of slowly growing mycobacteria by biochemical tests and lipid analyses. J. Clin. Microbiol. 30: 1972-1975, 1992. PubMed: 1500501
Picardeau M, Vincent V. Characterization of large linear plasmids in mycobacteria. J. Bacteriol. 179: 2753-2756, 1997. PubMed: 9098076