Photobacterium iliopiscarium (Onarheim et al.) Urakawa et al.
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2. Using a single tube of chilled #2 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a chilled #2 agar slant and/or plate.
5. Incubate the tubes and plate at 15°C for 48-72 hours.
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Validation list no. 53. Int. J. Syst. Bacteriol. 45: 418-419, 1995.
Onarheim AM, et al. Characterization and identification of two Vibrio species indigenous to the intestine of fish in cold sea water: description of Vibrio iliopiscarius sp. nov.. Syst. Appl. Microbiol. 17: 370-379, 1994.
Urakawa H, et al. Reassessment of the taxonomic position of Vibrio iliopiscarius (Onarheim et al. 1994) and proposal for Photobacterium iliopiscarium comb. nov.. Int. J. Syst. Bacteriol. 49: 257-260, 1999. PubMed: 10028270