Azoarcus tolulyticus Zhou et al.
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2. From a single tube of #1981 broth (5 to 6 ml), withdraw approximately 0.6 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Use 0.5ml of this suspension to inoculate a slant and 0.1ml to inoculate plates.
4. Incubate tubes and plates at 30oC, under aerobic conditions, for 48 hours.
Cells are Gram negative, short, thick rods.
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Zhou J, et al. Phylogenetic analyses of a new group of denitrifiers capable of anaerobic growth of toluene and description of Azoarcus tolulyticus sp. nov.. Int. J. Syst. Bacteriol. 45: 500-506, 1995. PubMed: 8590677
Fries MR, et al. Isolation, characterization, and distribution of denitrifying toluene degraders from a variety of habitats. Appl. Environ. Microbiol. 60: 2802-2810, 1994. PubMed: 8085824