Photobacterium damselae subsp. piscicida (Snieszko et al.) Gauthier et al.
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2. From a single tube of the recommended broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, an agar slant and/or a plate.
5. Incubate the tubes and plate at 26°C for 48 hours.
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Gauthier G, et al. Small-subunit rRNA sequences and whole DNA relatedness concur for the reassignment of Pasteurella piscicida (Snieszko et al.) Janssen and Surgalla to the genus Photobacterium as Photobacterium damsela subsp. piscicida comb. nov.. Int. J. Syst. Bacteriol. 45: 139-144, 1995. PubMed: 7531996