Brevibacillus formosus (Shida et al.) Shida et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 slant, and/or plate.
5. Incubate all tubes and plates at 30oC for 24 hours.
On Nutrient Agar plates, colonies are circular, flat, entire, smooth, glistening and translucent.
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Shida O, et al. Proposal of Bacillus reuszeri sp. nov., Bacillus formosus sp. nov., nom. rev., and Bacillus borstelensis sp. nov., nom. rev.. Int. J. Syst. Bacteriol. 45: 93-100, 1995.
Shida O, et al. Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen nov.. Int. J. Syst. Bacteriol. 46: 939-946, 1996. PubMed: 8863420