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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Incubate tubes and anaerobic plate under anaerobic conditions at 37oC. Incubate one blood plate aerobically at 37oC.
3. Within 24‑48 hours, growth should be evident by moderate turbidity in the broth. On agar surfaces, colonies are pinpoint, circular, and transparent with no hemolysis. No growth should occur on the blood agar plate incubated aerobically.
Storage at liquid nitrogen temperatures, with 10% glycerol as the cryoprotectant, is recommended for long-tern preservation.
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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