Pseudonocardia halophobica (Akimov et al.) McVeigh et al.
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2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #196 agar slant and/or plate.
5. Incubate the tubes and plate at 28oC for 2 to 5 days.
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Akimov VN, et al. Pseudoamycolata halophobica gen. nov., sp. nov.. Int. J. Syst. Bacteriol. 39: 457-461, 1989.
McVeigh HP, et al. The phylogenetic position of Pseudoamycolata halophobica (Akimov et al. 1989) and a proposal to reclassify it as Pseudonocardia halophobica. Int. J. Syst. Bacteriol. 44: 300-302, 1994. PubMed: 8186094