Lactobacillus pontis Vogel et al.
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2. From a single tube of #1936 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well
4. Use several drops of this suspension to inoculate a second tube of broth, a slant and/or a plate.
5. Incubate tubes and plate at 30oC in an atmosphere of 5-10% O2 and 10% CO2 for 48 hours. Loosen screw caps of all test tubes during the incubation period.
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Vogel RF, et al. Identification of lactobacilli from sourdough and description of Lactobacillus pontis sp. nov.. Int. J. Syst. Bacteriol. 44: 223-229, 1994. PubMed: 8186088