Flavobacterium hibernum McCammon et al.
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2. Using a single tube of #1913 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1913 agar slant and/or plate.
5. Incubate the tubes and plate at 20oC for 48 hours.
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McCammon SA, et al. Flavobacterium hibernum sp. nov., a lactose-utilizing bacterium from a freshwater Antarctic lake. Int. J. Syst. Bacteriol. 48: 1405-1412, 1998. PubMed: 9828443