Ornithobacterium rhinotracheale Vandamme et al.
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2. From a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate a second tube of broth, a slant, and/or a plate.
5. Incubate all tubes and plates under microaerophilic conditions at 35°C for 48 to 72 hours.
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Vandamme P, et al. Ornithobacterium rhinotracheale gen. nov., sp. nov., isolated from the avian respiratory tract. Int. J. Syst. Bacteriol. 44: 24-37, 1994. PubMed: 8123560