Arcobacter skirrowii Vandamme et al.
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2. Using a single tube of #1147 broth (5-6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or l.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a # 260 slant and/or plate.
5. Incubate tubes and plate at 35oC, under microaerophilic conditions, for 72 hours. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method. Incubate slant with cap loose.
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Vandamme P, et al. Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int. J. Syst. Bacteriol. 42: 344-356, 1992. PubMed: 1503968