Brevibacillus choshinensis (Takagi et al.) Shida et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24 hours.
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Takagi H, et al. Characterization of Bacillus brevis with descriptions of Bacillus migulanus sp. nov., Bacillus choshinensis sp. nov., Bacillus parabrevis sp. nov., and Bacillus galactophilus sp. nov.. Int. J. Syst. Bacteriol. 43: 221-231, 1993. PubMed: 8494737
Shida O, et al. Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen nov.. Int. J. Syst. Bacteriol. 46: 939-946, 1996. PubMed: 8863420