Mycobacterium alvei Ausina et al.
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2. From a single tube of the recommended broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or
1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Heavily inoculate a slant of #90 and/or a plate of #173 agar.
5. After growth has been achieved, additional transfers may be made.
Colonies on #173 plates are irregular, wrinkled, dry, and white.
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