Mycobacterium alvei Ausina et al.
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2. Using a single tube of #1395 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Heavily inoculate a #90 agar slant and/or plate of #173.
5. Incubate the tubes and plate at 30oC for at least one week.
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Ausina V, et al. Mycobacterium alvei sp. nov.. Int. J. Syst. Bacteriol. 42: 529-535, 1992. PubMed: 1390105
Luquin M, et al. Distribution of a novel mycolic acid in species of the genus Mycobacterium. Int. J. Syst. Bacteriol. 41: 390-394, 1991. PubMed: 1883713