Nocardiopsis lucentensis Yassin et al.
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2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #527 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 3-5 days.
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Yassin AF, et al. A new actinomycete species, Nocardiopsis lucentensis sp. nov.. Int. J. Syst. Bacteriol. 43: 266-271, 1993.