Neisseria meningitidis (Albrecht and Ghon) Murray
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2. Using a single tube of #814 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use 0.2 ml of the suspension to inoculate a #814 slant, and/or 0.1 ml for a plate.
5. Incubate all tubes and plate at 37oC with 5% CO2. Growth should occur in 24‑48 hours. Assure that screw caps are loose during incubation period.
Cells are large Gram negative bean-shaped cocci in pairs.
Brain Heart Infusion (BD 237500) can be used to rehydrate vial contents if #814 broth is not available, but it will not support growth as well as the GC broth.
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Ashton FE, et al. Emergence of a virulent clone of Neisseria meningitidis serotype 2a that is associated with meningococcal group C disease in Canada. J. Clin. Microbiol. 29: 2489-2493, 1991. PubMed: 1774254
human blood, Ontario