Amycolatopsis azurea (Omura et al.) Henssen et al.
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2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #196 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 3 to 5 days.
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Omura S, et al. Studies on bacterial cell wall inhibitors. VII. Azureomycins A and B, new antibiotics produced by Pseudonocardia azurea nov. sp. Taxonomy of the producing organism, isolation, characterization and biological properties. J. Antibiot. 32: 985-994, 1979. PubMed: 528377
Henssen A, et al. Transfer of Pseudonocardia azurea and "Pseudonocardia fastidiosa" to the genus Amycolatopsis, with emended species description. Int. J. Syst. Bacteriol. 37: 292-295, 1987.