Butyrivibrio fibrisolvens Bryant and Small
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2. Under anaerobic conditions, withdraw 0.5 ml of the appropriate medium from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. Additional tubes with 0.5 ml each from the suspension. Streak onto a blood plate and incubate aerobically to check for purity.
4. Incubate tubes under an anaerobic atmosphere at 37oC.
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Wachenheim DE, Patterson JA. Anaerobic production of extracellar polysaccharide by Butyrivibrio fibrisolvens nyx. Appl. Environ. Microbiol. 58: 385-391, 1992.