Campylobacter helveticus Stanley et al.
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2. Using a single tube of #1115 or #177 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #260 agar slant and/or plate.
1. Incubate tubes and plate at 37oC, under microaerophilic conditions, for 48 hours. Use an anaerobe jar with an active catalyst and a Campylobacter microaerophilic gas generator pack, or other acceptable method. Incubate slant with cap loose.
Fluid Thioglycollate tube may be incubated aerobically.
Storage at liquid nitrogen temperatures, with 10% sterile glycerol as the cryoprotectant, is recommended for long-term preservation.
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Stanley J, et al. Campylobacter helveticus sp. nov., a new thermophilic species from domestic animals: characterization, and cloning of a species-specific DNA probe. J Gen Microbiol 138: 2293-2303, 1992. PubMed: 1479353