Vagococcus salmoninarum Wallbanks et al.
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2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
5. Incubate all tubes and plate at 26°C for 24 hours.
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Wallbanks S, et al. 16S rRNA sequence determination for members of the genus Carnobacterium and related lactic acid bacteria and description of Vagococcus salmoninarum sp. nov.. Int. J. Syst. Bacteriol. 40: 224-230, 1990. PubMed: 1697764