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Shewanella algae Simidu et al. emend. Nozue

51181

Product category
Bacteria
Strain designation
BrY
Type strain
No
Isolation source
Sediment from Great Bay estuary
Geographical isolation
United States; New Hampshire
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Reduces iron (ferric, FeIII) ferric iron
Resistant to chromium
Biotechnology
Preceptrol
No

Handling information

Medium
Temperature
30°C
Atmosphere
Aerobic
Handling procedure

  1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #2 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #2 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #2 plate and/or #2 agar slant.
  4. Incubate at 30°C for 24 hours.

Handling notes

To demonstrate anaerobic growth by dissimilatory iron (III) reduction, use ATCC Medium #1931 broth.

 

1931 Broth:  Ferric citrate Medium

Ferric citrate, 13.7 g

NaHCO3, 2.5 g

KCl, 0.1 g

NH4Cl, 1.5 g

NaH2PO4, 0.6 g

Wolfe's Vitamin Solution (see below), 10.0 mL

Wolfe's Mineral Solution (see below), 10.0 mL

Sodium lactate, 30.0 mM

Distilled water, 1.0 L

Boil ferric citrate in distilled water to dissolve; cool to room temperature.  Adjust to pH 6.6 with 10N NaOH.  Add remaining components.  Equilibrate medium under 80% N2, 20% CO2.  Dispense medium anaerobically under same gas phase.  Autoclave anaerobically at 121oC for 15 minutes.  Final pH 7.0.

 

Wolfe's Vitamin Solution:

Biotin, 2.0 mg

Folic acid, 2.0 mg

Pyridoxine HCl, 10.0 mg

Thiamine HCl, 5.0 mg

Riboflavin, 5.0 mg

Nicotinic acid, 5.0 mg

Calcium D-(+)-pantothenate, 5.0 mg

Vitamin B12, 0.1 mg

p-Aminobenzoic acid, 5.0 mg

Thioctic acid, 5.0 mg

Distilled water, 1.0 L

Wolfe's Mineral Solution:

Nitrilotriacetic acid, 1.5 g

MgSO4.7H2O, 3.0 g

MnSO4.H2O, 0.5 g

NaCl, 1.0 g

FeSO4.7H2O, 0.1 g

CoCl2.6H2O, 0.1 g

CaCl2, 0.1 g

ZnSO4.7H2O, 0.1 g

CuSO4.5H2O, 0.01 g

AlK(SO4)2.12H2O, 0.01 g

H3BO3, 0.01 g

Na2MoO4.2H2O, 0.01 g

Distilled water, 1.0 L

Add nitrilotriacetic acid to approximately 500 mL of water and adjust to pH 6.5 with KOH to dissolve the compound. Bring volume to 1.0 L with remaining water and add remaining compounds one at a time.

 

Additional information on this culture is available on the ATCC web site at www.atcc.org.

History

Deposited as
Shewanella algae Simidu et al. emend. Nozue
Depositors
F Caccavo
Type of isolate
Environmental
Cross references
GenBank AF005691 Shewanella alga DNA gyrase beta-subunit (gyrB) gene, partial cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Caccavo F Jr., et al. A hydrogen-oxidizing, Fe(III)-reducing microorganism from the Great Bay Estuary, New Hampshire. Appl. Environ. Microbiol. 58: 3211-3216, 1992.

Caccavo F Jr., et al. Deflocculation of activated sludge by the dissimilatory Fe(III)-reducing bacterium Shewanella alga BrY. Appl. Environ. Microbiol. 62: 1487-1490, 1996.

Caccavo F, McInerney MJ. Method for bacterial reduction of chromium (VI). US Patent 5,569,596 dated Oct 29 1996

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