Aeribacillus pallidus (Scholz et al.) Minana-Galbis et al.
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2. Using a single tube of #1812 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1812 agar slant and/or plate.
5. Incubate the tubes and plate at 55°C for 24 hours.
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Scholz T, et al. Bacillus pallidus sp.nov., a new thermophilic species from sewage. Syst. Appl. Microbiol. 9: 91-96, 1987.
Validation list no. 24. Int. J. Syst. Bacteriol. 38: 136-137, 1988.