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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Culture conditions encouraging slow growth are important in maintaining the sheath. This can be achieved by lowering the incubation temperature (20oC rather than 26oC) and by incubating broth cultures statically.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
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Emerson D, Ghirose WC. Isolation, cultural maintenance, and taxonomy of a sheath-forming strain of Leptothrix discophora and characterization of manganese-oxidizing activity associated with the sheath. Appl. Environ. Microbiol. 58: 4001-4010, 1992.
Emerson D, Ghiorse WC. Ultrastructure and chemical composition of the sheath of Leptothrix discophora SP-6. J. Bacteriol. 175: 7808-7818, 1993. PubMed: 7504663
Emerson D, Ghiorse WC. Role of disulfide bonds in maintaining the structural integrity of the sheath of Leptothrix discophora SP-6. J. Bacteriol. 175: 7819-7827, 1993. PubMed: 8253670