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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Anaerobic conditions for transfer may be obtained by either of the following:
Anaerobic conditions for incubation may be obtained by any of the following:
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
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Hudson MJ, et al. Diagnosis of swine dysentery: spirochaetes which may be confused with Treponema hyodysenteriae. Vet. Rec. 99: 498-500, 1976. PubMed: 1014306
Validation list no. 64. Int. J. Syst. Bacteriol. 48: 327-328, 1998.
Stanton TB, et al. Recognition of two new species of intestinal spirochetes: Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov.. Int. J. Syst. Bacteriol. 47: 1007-1012, 1997.