Hartmannella vermiformis Page
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Transfer approximately 0.25 ml to a fresh T-25 tissue culture flask containing 5 ml of fresh ATCC medium 1034.
3. Screw the caps on tightly and incubate at 25°C.
4. The amoebae will form an almost continuous sheet of cells on the bottom surface of the flask. Repeat steps 1-3 at 10-14 d intervals.
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Wadowsky RM, et al. Gentamicin-containing peptone-yeast extract medium for cocultivation of Hartmannella veriformis ATCC 50256 and virulent strains of Legionella pneumophila. Appl. Environ. Microbiol. 61: 4464-4467, 1995.
Wadowsky RM, et alMultiplication of virulent and antivirulent strains of Legionella pneumophila in cultures of Hartmannella vermiformisIn: Wadowsky RM, et alLegionella: current status and emerging perspectivesWashington, DCAmerican Society for Microbiologypp. 145-147, 1993