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Hartmannella vermiformis Page

50237

Product category
Protists
Classification
Amoebozoa, Tubulinea, Tubulinida
Strain designation
CDC-19
Type strain
No
Isolation source
Hospital cooling tower drain
Geographical isolation
United States; South Dakota
Product format
Frozen
Storage conditions
-80°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
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Price: $235.00 EA
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Modified PYNFH Medium [ATCC medium 1034]

327-X

Price: $74.00 ea

Detailed product information

General

Specific applications
Interaction of a pathogen and its natural host

Characteristics

Comments
Nature of phagosome containing Legionella pneumophila
Enhances Legionella lung infection in murine model
Protein expression with bacterial parasite Legionella pneumophila
Medium for cocultivation with Legionella pneumophila
Interaction of a pathogen and its natural host

Handling information

Medium
Instruction for complete medium
ATCC Medium 1034 (ATCC Medium 1034 is available in a freeze-dried format as cat. no. 327-X)
Temperature
25°C
Culture system
Axenic
Handling procedure
Storage and Culture Initiation

Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.  If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week.  Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C).  Storage of frozen material at this temperature will result in the death of the culture.

  1. To thaw a frozen ampule, place it in a 35°C water bath until thawed (2 to 3 min).  Immerse the ampule just sufficiently to cover only the frozen material.  Do not agitate the ampule.
  2. Immediately after thawing, aseptically transfer contents to either a plastic screw-capped tube containing 5 mL ATCC medium 1034, or to a T-25 tissue culture flask containing 10 mL ATCC medium 1034.  Incubate at 25°C with the cap screwed on tightly (incubate a test tube on a 15° horizontal slant).
Culture maintenance
  1. Vigorously agitate a culture at or near peak density and aseptically transfer 0.1 ml to 0.2 ml to a fresh tube or flask of ATCC medium 1034.
  2. Incubate at 25°C with the cap screwed on tightly (incubate a test tube on a 15° horizontal slant).
Cryopreservation
  1. Harvest cells from a culture that is at or near peak density by centrifugation at 600 x g for 5 min. Pool the cell pellets into a single tube.
  2. Adjust the concentration of cells to 2.0 x 106/mL.  If the concentration is too low, centrifuge at 600 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. Prepare a 15% (v/v) sterile DMSO solution in ATCC medium 1034 as follows:
    1. Add the required volume of DMSO to a glass screw-capped test tube and place on ice. Allow the DMSO to solidify. 
    2. Add the required volume of refrigerated ATCC medium 1034.  Dissolve the DMSO by inverting several times.  If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.
  4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the start of the freezing process should be no less than 15 min and no longer than 60 min.
  5. Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.
  7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just sufficiently to cover only the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into a fresh tube or flask of ATCC medium 1034.
  10. Incubate at 25°C with the cap screwed on tightly (incubate a test tube on a 15° horizontal slant).

History

Deposited as
Hartmannella vermiformis Page
Depositors
BS Fields
Special collection
NSF - Protistology

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

abu Kwaik Y, et al. Protein expression by the protozoan Hartmannella vermiformis upon contact with its bacterial parasite Legionella pneumophila. Infect. Immun. 62: 1860-1866, 1994. PubMed: 8168950

Abu Kwaik Y, et al. Signal transduction in the protozoan host hartmannella vermiformis upon attachment and invasion by legionella micdadei. Appl. Environ. Microbiol. 64: 3134-3139, 1998. PubMed: 9726850

Aragon V, et al. Legionella pneumophila major acid phosphatase and its role in intracellular infection. Infect. Immun. 69: 177-185, 2001. PubMed: 11119504

Aragon V, et al. Secreted enzymatic activities of wild-type and pilD-deficient Legionella pneumophila. Infect. Immun. 68: 1855-1863, 2000. PubMed: 10722574

Brieland J, et al. Coinoculation with Hartmannella vermiformis enhances replicative Legionella pneumophila lung infection in a murine model of Legionnaires' disease. Infect. Immun. 64: 2449-2456, 1996. PubMed: 8698466

View All Curated Citations for this Product

Frequently Asked Questions

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