Plasmodium berghei Vincke and Lips
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Storage and Culture Initiation
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
The following directions for recovery from the frozen state must be carefully followed if a culture is to be successfully established.
To monitor the infection (recommended every 24 hrs. from day 3 onwards), withdraw a small amount of blood (0.05–0.1ml) from a limb using a hemolet and make a smear (see below). When parasitemia reaches 10–30%, parasites should be harvested.
Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust.
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Boyer JM, et al. Lipid composition and activity of a lytic factor isolated from Plasmodium berghei. Infect. Immun. 25: 805-809, 1979. PubMed: 387594
D'Antonio LE, et al. Malaria vaccine antigen(s): detergent solubilization, partial isolation, and recovery of immunoprotective activity. Infect. Immun. 43: 442-444, 1984. PubMed: 6360908
Alger NE, et al. Plasmodium berghei NK65 in the inbred A-J mouse: variations in virulence of P. berghei demes. J. Protozool. 18: 598-601, 1971. PubMed: 5133123
Alger NE, Keshavarz-Valian H. Plasmodium berghei: the effects of suppressor factor on vaccination. Int. J. Parasitol. 14: 301-307, 1984. PubMed: 6381347
D'Antonio LE, et al. Model system for study of artificially induced resistance to malaria. Nature 223: 507-509, 1969. PubMed: 5796953