Plasmodium falciparum Welch
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
In vitro culture in human erythrocyte
Storage and Culture InitiationFrozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
The following directions for recovery from the frozen state must be carefully followed if a culture is to be successfully established.
* Medium Formulation:
NOTE: Outdated blood and serum cannot be used to cultivate this strain. Use only fresh blood and serum.
Making a Blood Smear:
Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.
** To formulate glycerolyte medium, combine the following with distilled water to 100 ml: 57.00g glycerol, 1.60g sodium lactate (C3H5NaO3), 30.00mg potassium chloride (KCl), 1.38g sodium dihydrogen phosphate (NaH2PO4). Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave to sterilize, and store at 4°C.
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Bhasin VK, Trager W. Gametocyte-forming and non-gametocyte-forming clones of Plasmodium falciparum. Am. J. Trop. Med. Hyg. 33: 534-537, 1984. PubMed: 6383092