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Plasmodium falciparum Welch

50028

Product category
Protists
Product type
Parasitic protozoan
Classification
KINGDOM: Protozoa
Strain designation
FCR-8/West African
Type strain
No
Isolation source
West Africa
Geographical isolation
Africa
Applications
Infectious disease research
Vector-borne disease research
Product format
Frozen
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Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Emerging infectious disease research
Vector borne research

Characteristics

Susceptibility profile
Susceptible
Chloroquine
Comments
Chloroquine sensitive.

Handling information

Medium
Instruction for complete medium
ATCC Medium 2196 and type O blood
Temperature
37°C
Incubation
In vitro culture in human erythrocytes
Culture maintenance
Changing of the culture medium every 24 hours is required for a malaria-infected erythrocyte culture.  Add washed, uninfected red blood cells (RBCs) to 1-3% haematocrit, and maintain parasitemia at 2-3% for continuous culture.

1.     Remove flask with infected culture from 37oC incubator and place onto flask warmer in biological safety hood.

2.     Carefully aspirate the medium with a sterile unplugged Pasteur pipet attached to a vacuum line. Remove as much fluid as possible without taking the cells.

3.     Aseptically add sterile warm (37oC) completed medium to the flask (~8ml to a T-25, ~25ml to a T-75, etc.).  Mix and smear as required to determine parasitemia (see below).

4.     Add washed RBCs as necessary to obtain desired haematocrit and parasitemia.

5.     Gently mix and aerate culture with gas mixture of 5% CO2, 5% O2 and 90% N2 using a sterile, cotton plugged Pasteur pipet. Quickly tighten cap of the flask and place in 37oC incubator until the next medium change.

Cryopreservation
Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.

1.     Centrifuge ring-stage culture for 5 mins. at 1000 x g in 50 ml centrifuge tube.

2.     Aspirate supernatant using sterile Pasteur pipet.

3.     Resuspend pellet gently in remaining supernatant.

4.     Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:

        A.    Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.

B.    Add the remaining 4 volumes of glycerolyte and gently agitate.

5.   Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.).

6.   Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor.

       

History

Deposited as
Plasmodium falciparum Welch
Depositors
W Trager
Chain of custody
ATCC <-- W Trager <-- P. Nguyen-Dinh
Type of isolate
Human
Year of origin
1978

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Nguyen-Dinh P, Trager W. Plasmodium falciparum in vitro: determination of chloroquine sensitivity of three new strains by a modified 48-hour test. Am. J. Trop. Med. Hyg. 29: 339-342, 1980. PubMed: 6992605

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