Enterococcus flavescens Pompei et al.
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2. From a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, a #44 agar slant and/or a plate.
5. Incubate the tubes and plate at 37oC for 24 hours.
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Pompei R, et al. Enterococcus flavescens sp. nov., a new species of enterococci of clinical origin [published erratum appears in Int. J. Syst. Bacteriol. 43: 190, 1993]. Int. J. Syst. Bacteriol. 42: 365-369, 1992. PubMed: 1503970
Pompei R, et al. Characterization of yellow-pigmented enterococci from severe human infections [published erratum appears in J. Clin. Microbiol. 30: 3302, 1992]. J. Clin. Microbiol. 29: 2884-2886, 1991. PubMed: 1757566