Listeria ivanovii subsp. londoniensis Boerlin et al.
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2. From a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, a #44 agar slant and/or a plate.
5. Incubate the tubes and plate at 37°C for 24 hours.
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Boerlin P, et al. Listeria ivanovii subsp. londoniensis subsp. nov.. Int. J. Syst. Bacteriol. 42: 69-73, 1992.