Staphylococcus muscae Hajek et al.
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2. From a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Incubate the tube at 37°C for 24 hours.
5. After growth has been achieved, use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
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Hajek V, et al. Staphylococcus muscae, a new species isolated from flies. Int. J. Syst. Bacteriol. 42: 97-101, 1992. PubMed: 1371067
house fly, Musca domestica