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Mycoplasma genitalium Tully et al.


Product category
Strain designation
Type strain
Isolation source
Synovial fluid of arthritic knee joint
Geographical isolation
United States; Texas
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information




Identified in Arch. Intern. Med. 148: 969-970, 1988 as Myclplasma pneumoniae; later found to be a mixture of M. pneumoniae and M. genitalium.
Case report

Handling information

Broth: Aerobic
Plates: 5% CO2
Handling procedure
  1. Open vial according to enclosed instructions or visit for instructions.
  2. Rehydrate the entire pellet with approximately 0.25 mL of #988 broth. Aseptically transfer the entire contents to a 25 cm2 T-flask with 2.5 mL of 988 broth.
  3. Additional flasks can be inoculated by transferring 0.25 mL of the primary T-flask to these secondary flasks. 
  4. Incubate the flasks in a horizontal position at 37°C for 7-14 days. Plates incubate at 37°C for 7-14 days in an atmosphere of 5% CO2
  5. Growth is indicated by a medium color change from red to yellow. Growth can be observed as a thin film of adherent cells to the bottom of the T-flask and/ or observe for flocs in the medium.
  6. When the medium has turned orange, loosen adherent cells with a suitable, sterile scraper. Aseptically transfer the suspension to a centrifuge tube. Spin at 9000 rpm for 30 minutes. Pour off supernatant and resuspend pellet in fresh medium. You should have enough inoculum to make four additional small flasks, or one large one.
Handling notes

Commercially available SP4 Glucose (Remel catalog # R112585 or #R20376 for broth, and Remel catalog # R20276 for agar) may yield the best growth.

The flasks should be checked periodically to ensure the media is not evaporating. Add media as needed to maintain the 2.25 mL volume.

Additional information on this culture is available on the ATCC® web site at


Deposited as
Mycoplasma genitalium Tully et al.
JG Tully
Chain of custody
ATCC <-- JG Tully <-- G. Buck UTMB-10G
Type of isolate

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Davis CP, et al. Isolation of Mycoplasma pneumoniae from synovial fluid samples in a patient with pneumonia and polyarthritis. Arch. Intern. Med. 148: 969-970, 1988. PubMed: 3128197

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