Mycoplasma genitalium Tully et al.
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Commercially available SP4 Glucose (Remel catalog # R112585 or #R20376 for broth, and Remel catalog # R20276 for agar) may yield the best growth.
The flasks should be checked periodically to ensure the media is not evaporating. Add media as needed to maintain the 2.25 mL volume.
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Davis CP, et al. Isolation of Mycoplasma pneumoniae from synovial fluid samples in a patient with pneumonia and polyarthritis. Arch. Intern. Med. 148: 969-970, 1988. PubMed: 3128197