Mycobacterium avium subsp. silvaticum Thorel et al.
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2. From a single tube of the recommended broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Heavily inoculate a slant and/or a plate of #1507 agar.
5. After growth has been achieved, additional transfers may be made.
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Thorel MF, et al. Numerical taxonomy of mycobactin-dependent mycobacteria, emended description of Mycobacterium avium, and description of Mycobacterium avium subsp. avium subsp. nov., Mycobacterium avium subsp. paratuberculosis subsp. nov., and Mycobacterium avium subsp.silvaticum subsp. nov.. Int. J. Syst. Bacteriol. 40: 254-260, 1990. PubMed: 2397193