Laceyella sacchari (Lacey) Yoon et al.
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2. Using a single tube of #196 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #196 slant, and/or plate.
5. Incubate all tubes and plates at 50oC for 24 to 72 hours.
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Validation list no. 31. Int. J. Syst. Bacteriol. 39: 495-497, 1989.
Yoon JH, et al. DNA--DNA relatedness among Thermoactinomyces species: Thermoactinomyces candidus as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus as a synonym of Thermoactinomyces sacchari. Int. J. Syst. Evol. Microbiol. 50: 1905-1908, 2000. PubMed: 11034503
type strain of Thermoactinomyces thalpophilus
Yoon JH, Kim IG, Shin YK, Park YH. Proposal of the genus Thermoactinomyces sensu stricto and three new genera, Laceyella, Thermoflavimicrobium and Seinonella, on the basis of phenotypic, phylogenetic and chemotaxonomic analyses. Int. J. Syst. Evol. Microbiol. 55(Pt 1): 395-400, 2005. PubMed: 15653907