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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #196 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #196 slant, and/or plate.
5. Incubate all tubes and plates at 50oC for 24 to 72 hours.
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Validation list no. 31. Int. J. Syst. Bacteriol. 39: 495-497, 1989.
Yoon JH, et al. DNA--DNA relatedness among Thermoactinomyces species: Thermoactinomyces candidus as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus as a synonym of Thermoactinomyces sacchari. Int. J. Syst. Evol. Microbiol. 50: 1905-1908, 2000. PubMed: 11034503
type strain of Thermoactinomyces thalpophilus
Yoon JH, Kim IG, Shin YK, Park YH. Proposal of the genus Thermoactinomyces sensu stricto and three new genera, Laceyella, Thermoflavimicrobium and Seinonella, on the basis of phenotypic, phylogenetic and chemotaxonomic analyses. Int. J. Syst. Evol. Microbiol. 55(Pt 1): 395-400, 2005. PubMed: 15653907