Carnobacterium funditum Franzmann et al.
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2. From a single tube of #1823 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well
4. Use several drops of this suspension to inoculate a second tube of broth, a slant and/or a plate.
5. Incubate tubes and plate at 20oC for 24 to 48 hours.
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Franzmann PD, et al. Psychrotrophic, lactic acid-producing bacteria from anoxic waters in Ace Lake, Antarctica; Carnobacterium funditum sp. nov. and Carnobacterium alterfunditum sp. nov.. Arch. Microbiol. 156: 255-262, 1991. PubMed: 1793333
Validation list no. 44. Int. J. Syst. Bacteriol. 43: 188-189, 1993.