Bacillus thermocloacae Demharter and Hensel
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2. Using a single tube of #1812 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1812 slant, and/or plate.
5. Incubate all tubes and plates at 60oC for 48-72 hours.
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Validation list no. 31. Int. J. Syst. Bacteriol. 39: 495-497, 1989.
Demharter W, Hensel R. Bacillus thermocloaceae sp.nov., a new thermophilic species from sewage sludge. Syst. Appl. Microbiol. 11: 272-276, 1989.