Rhizobium vitis (Ophel and Kerr) Young et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 26°C for 48 to 72 hours.
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Ophel K, Kerr A. Agrobacterium vitis sp. nov. for strains of Agrobacterium biovar 3 from grapevines. Int. J. Syst. Bacteriol. 40: 236-241, 1990.
Young JM, et al. A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new combinations: Rhizobium radiobacter, R. rhizogenes, R. rubi, R. undicola and R. vitis. Int. J. Syst. Evol. Microbiol. 51: 89-103, 2001. PubMed: 11211278
grapevine, Vitis vinifera