Bacillus amyloliquefaciens (Fukumoto) Priest et al.
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2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24-48 hours.
1. Smooth, entire, glistening, and 1 mm
2. Erose, rough, flat and 2-3 mm
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Roberts RJ, et al. Recognition sequence of specific endonuclease BamH.I from Bacillus amyloliquefaciens H. Nature 265: 82-84, 1977. PubMed: 834250
Hattman S, et al. Sequence specificity of DNA methylases from Bacillus amyloliquefaciens and Bacillus brevis. J. Mol. Biol. 124: 701-711, 1978. PubMed: 712853
Wilson GA, Young FE. Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H. J. Mol. Biol. 97: 123-125, 1975. PubMed: 1177312