Bacillus subtilis subsp. subtilis (Ehrenberg) Cohn
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2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
5. Incubate the tubes and plate at 30oC for 24 hours.
Cells are Gram positive, motile, medium-length rods with rounded ends, arranged singly and in pairs. Oval spores are produced.
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