Cutibacterium avidum Scholz and Kilian
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2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and one plate under anaerobic conditions at 37°C. Incubate one blood plate aerobically at 37°C.
5. Growth should be detected within 24-48 hours.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
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Goodsell ME, et al. Two types of Propionibacterium avidum with different isomers of diaminopimelic acid. Curr. Microbiol. 22: 225-230, 1991.
Cummins CS. Identification of Propionibacterium acnes and related organisms by precipitin tests with trichloroacetic acid extracts. J. Clin. Microbiol. 2: 104-110, 1976. PubMed: 972178
Gross CS, et al. Electrophoretic protein patterns and enzyme mobilities in anaerobic coryneforms. Appl. Environ. Microbiol. 35: 1102-1108, 1978. PubMed: 677876
Johnson JL, Cummins CS. Cell wall composition and deoxyribonucleic acid similarities among the anaerobic coryneforms, classical propionibacteria, and strains of Arachnia propionica. J. Bacteriol. 109: 1047-1066, 1972. PubMed: 5062339