Legionella lansingensis Thacker et al.
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2. Using a single tube of #1099 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1099 agar slant and/or plate.
5. Incubate the tubes and plate at 37oC 5% CO2 for 48 hours.
Before rehydration, vials may be stored at +2 to +8oC for extended periods with minimal loss of viability. Subculturing to maintain culture is discouraged because of the possibility of mutation, selection of variants, or contamination. To minimize changes, it is recommended that cells be harvested and stored at ‑70oC or below.
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Validation list no. 50. Int. J. Syst. Bacteriol. 44: 595, 1994.
Thacker WL, et al. Legionella lansingensis sp. nov. isolated from a patient with pneumonia and underlying chronic lymphocytic leukemia. J. Clin. Microbiol. 30: 2398-2401, 1992. PubMed: 1401005