Acidovorax temperans Willems et al.
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2. From a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate the tubes and plate at 30°C for 48 hours.
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Willems A, et al. Acidovorax, a new genus for Pseudomonas facilis, Pseudomonas delafieldii, E. Falsen (EF) group 13, EF group 16, and several clinical isolates, with the species Acidovorax facilis comb. nov., Acidovorax delafieldii comb. nov., and Acidovorax temperanssp. nov. Int. J. Syst. Bacteriol. 40: 384-398, 1990. PubMed: 2275854