Acidipropionibacterium acidipropionici (Orla-Jensen) Scholz et Kilian
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2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and one plate under anaerobic conditions at 30°C. Incubate one blood plate aerobically at 30°C.
5. Within 48 hours, colonies are pinpoint, circular, entire, convex, white, translucent, glistening, and smooth. Growth can occur on the blood agar plates incubated aerobically.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Gibian H, et al. Production of optically active antipodes. US Patent 3,562,112 dated Feb 9 1971
Werkman CH, Brown RW. The propionic acid bacteria. II. Classificiation. J. Bacteriol. 26: 393-417, 1933.
Hitchner ER. Some physiological characteristics of the propionic acid bacteria. J. Bacteriol. 28: 473-479, 1934.
Hitchner ER. A cultural study of the propionic-acid bacteria. J. Bacteriol. 23: 40-41, 1932.
Johnson JL, Cummins CS. Cell wall composition and deoxyribonucleic acid similarities among the anaerobic coryneforms, classical propionibacteria, and strains of Arachnia propionica. J. Bacteriol. 109: 1047-1066, 1972. PubMed: 5062339