Pseudomonas aeruginosa (Schroeter) Migula
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2. Using a single tube of #1549 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Several drops of this suspension may be used to inoculate a #1549 agar slant and/or plate, but this strain may require a primary growth period in the initial broth before it will grow on any subculture attempts.
5. Incubate the primary broth tube and/or additional media at 28oC for 24-48 hours.
6. Within 24-48 hours, growth should be evident by turbidity in the primary broth. Secondary agar media and broth may be inoculated with this cell suspension.
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Higson FK, Focht DD. Degradation of 2-bromobenzoic acid by a strain of Pseudomonas aeruginosa. Appl. Environ. Microbiol. 56: 1615-1619, 1990. PubMed: 2116758