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Streptococcus pneumoniae (Klein) Chester

49619

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Used as a quality control organism for CLSI susceptibility testing, Vitek Compact GP card, Vitek Compact AST GP card as well as a quality control strain for Sensitire products.
Product category
Bacteria
Product type
Preceptrol culture
Strain designation
262 [CIP 104340]
Type strain
No
Genome sequenced strain
Yes
Isolation source
Sputum
Geographical isolation
United States; Arizona; Phoenix
Applications
Bioinformatics
Infectious disease research
Quality control
Respiratory disease research
Product format
Frozen
Storage conditions
-80°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Respiratory research
Quality control strain Ref BD Phoenix Streptococcus SMIC/ID QC Set. BD Becton Dickinson; Phoenix  Ref Sensititre™ H. influenzea/S. pneumoniae MIC Susceptibility. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems.
Susceptibility disc testing Ref Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9. Ref Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M42-A. Ref Methods for Antimicrobial Dilution and Disk susceptibility Testing of Infrequently Isolated or Fastidious Bacteria: Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M45-A. Ref Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M49-A.
Susceptibility testing Ref Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8. Ref Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3. Ref Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007 Ref Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P. Ref Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution;British Standard BS EN ISO 20776-1:2006.
Preceptrol
Yes

Characteristics

Serotype
19 (Danish designation 19F)
Comments
MIC values using HTM medium RefJorgensen JH, et al. Multicenter evaluation of the use of Haemophilus test medium for broth microdilution antimicrobial susceptibility testing of Streptococcus pneumoniae and development of quality control limits. J. Clin. Microbiol. 30: 961-966, 1992. PubMed: 1572984

Handling information

Medium
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
  1. Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
  2. Aseptically transfer the entire contents to a 5-6 mL tube of #44 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
  3. Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
  4. Incubate at 37°C for 24 hours in an atmosphere of 5% CO2.
Handling notes
An additional transfer may be required after initial 24 hours of growth in order to fully remove cyroprotectant and achieve acceptable growth.
Depositor-provided information: serotype 19F (Danish), type 19 (U.S.).
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Streptococcus pneumoniae (Klein) Chester
Depositors
JH Jorgensen
Chain of custody
ATCC <-- JH Jorgensen <-- M Saubolle
Type of isolate
Human
Patient age
75 years
Patient gender
Male
Cross references
GenBank Z74778 S.pneumoniae gene for dihydrofolate reductase.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Jorgensen JH, et al. Multicenter evaluation of the use of Haemophilus test medium for broth microdilution antimicrobial susceptibility testing of Streptococcus pneumoniae and development of quality control limits. J. Clin. Microbiol. 30: 961-966, 1992. PubMed: 1572984

Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3.

View All Curated Citations for this Product

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