Legionella fairfieldensis Thacker et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a pipette, withdraw the thawed culture from the vial and inoculate a single tube (5.0 to 6.0 ml) of ATCC Medium #1099 broth.
3. Use several drops of this suspension to inoculate a #1099 agar slant and/or plate.
4. Incubate at 37oC in an atmosphere of 5% CO2 for 48-72 hours.
Legionella species are nutritionally fastidious and are inhibited by media of poor caliber, so strict attention should be paid to the quality of the ingredients. The pH of the medium should be checked when cool, and is most critical. Unbuffered medium should not be used. Exposure of medium to light, particularly when hot, may also result in the accumulation of peroxides that can be inhibitory to the bacteria.
Subculturing to maintain culture is discouraged because of the possibility of mutation, selection of variants, or contamination. To minimize changes, it is recommended that cells be harvested and stored at ‑70oC or below.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Thacker WL, et al. Legionella fairfieldensis sp. nov. isolated from cooling tower waters in Australia. J. Clin. Microbiol. 29: 475-478, 1991. PubMed: 2037664
Deed RW, et al. Validation list no. 39. Int. J. Syst. Bacteriol. 41: 580-581, 1991. PubMed: 1742202