Ralstonia syzygii (Roberts et al.) Vaneechoutte et al.
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2. Using a single tube of #1099 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an agar slant and/or plate.
5. Incubate all tubes and plate at 28oC for 48-72 hours.
Before rehydration, vials may be stored at +2 to +8oC for extended periods with minimal loss of viability. Subculturing to maintain culture is discouraged because of the possibility of mutation, selection of variants, or contamination. To minimize changes, it is recommended that cells be harvested and stored at ‑70oC or below.
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Roberts SJ, et al. Pseudomonas syzygii, sp.nov., the cause of Sumatra disease of cloves. Syst. Appl. Microbiol. 13: 34-43, 1990.
Validation list no. 34. Int. J. Syst. Bacteriol. 40: 320-321, 1990.
Vaneechoutte M, et al. Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov.. Int. J. Syst. Evol. Microbiol. 54: 317-327, 2004. PubMed: 15023939
Diseased clove tree, Syzygium aromaticum, West Sumatra