Flavobacterium saccharophilum (ex Agbo and Moss) Bernardet et al.
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2. Using a single tube of #1753 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #1753 agar slant and/or a plate.
5. Incubate all tubes and plate at 26°C for 48 hours.
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Validation list no. 31. Int. J. Syst. Bacteriol. 39: 495-497, 1989.
Agbo JA, Moss MO. The isolation and characterization of agarolytic bacteria from a lowland river. J. Gen. Microbiol. 115: 355-368, 1979.
Bergey's Manual Syst. Bacteriol. 3: 2039-2040, 1989.
Bernardet JF, et al. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int. J. Syst. Bacteriol. 46: 128-148, 1996.