Flavobacterium psychrophilum (Borg) Bernardet et al.
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2. Using a single tube of #1751 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1751 agar slant and/or plate.
5. Incubate the tubes and plate at 18°C for 72 hours.
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Bernardet JF, Grimont PA. Deoxyribonucleic acid related ness and phenotypic characterization of Flexibacter columnaris sp. nov., nom. rev., Flexibacter psychrophilus sp. nov., nom. rev., and Flexibacter maritimus Wakabayashi, Hikida, and Masumura. Int. J. Syst. Bacteriol. 39: 346-354, 1989.
Bernardet JF, Kerouault B. Phenotypic and genomic studies of "Cytophaga psychrophila" isolated from diseased rainbow trout (Oncorhynchus mykiss) in France. Appl. Environ. Microbiol. 55: 1796-1800, 1989. PubMed: 2764577